Isolation and Pathogenicity of a Chinese Porcine Astrovirus Type 5 Strain HNPDS-01 and Its Influence on Cecum Microbiota in Piglets

Figure 6

Histopathological and immunohistochemical analysis of PAstV 5 -HNPDS-01 challenged piglets. (a–f) Piglets intestinal tissues (ileum, cecum, and colon) with typical histological lesions observed by hematoxylin–eosin (H&E) staining, scale bar is 200 μm. (g) Number of glands in large intestine per/square millimeter. (h–k) Piglets intestinal tissues (ileum) tested PAstV 5 specific monoclonal antibody, arrows indicate detection of PAstV 5 antigen in tissues, the scale bar is 200 μm in (h) and (i), the scale bar is 200 μm in (j) and (k).