Isolation and Pathogenicity of a Chinese Porcine Astrovirus Type 5 Strain HNPDS-01 and Its Influence on Cecum Microbiota in Piglets

Table 2

Amino acid divergence between the sequence (4,344−5,734 nt) of PAstV 5-HNPDS-01 strain and the suspected recombinant parental sequence.

Amino acid sites

58

110

114

178

181

186

189

191

197

206

209

213

214

221

224

PAstV 5 HNPDS-01 (OQ781001)

V

G

M

R

S

K

A

E

N

S

R

L

L

Q

H

PoAstV-VIRES-JL01 (MK378540)

I

G

L

K

N

T

P

H

N

T

K

A

A

Q

—

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

V

S

L

R

S

T

P

E

K

S

K

P

P

P

—

Amino acid sites

225

226

227

229

230

244

245

246

249

250

265

267

276

278

279

PAstV 5 HNPDS-01 (OQ781001)

H

L

T

S

P

I

K

V

S

E

I

V

N

V

D

PoAstV-VIRES-JL01 (MK378540)

—

V

T

S

P

V

R

V

S

G

M

A

T

I

N

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

—

S

A

T

T

I

K

T

A

E

M

V

T

V

N

Amino acid sites

280

281

282

285

288

290

298

303

305

306

307

311

313

315

321

PAstV 5 HNPDS-01 (OQ781001)

A

A

S

G

I

A

N

T

L

S

A

E

K

D

M

PoAstV-VIRES-JL01 (MK378540)

A

S

T

A

V

T

N

S

I

A

P

D

N

D

I

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

L

A

E

G

I

A

D

S

L

A

A

D

K

N

M

Amino acid sites

327

329

332

333

336

340

341

344

346

348

350

352

353

358

359

PAstV 5 HNPDS-01 (OQ781001)

V

L

G

T

S

V

V

S

S

A

I

A

S

T

Y

PoAstV-VIRES-JL01 (MK378540)

A

V

A

S

A

P

D

N

T

S

L

T

T

P

Y

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

V

V

A

S

A

P

T

N

T

A

L

T

T

P

S

Amino acid sites

360

361

362

363

364

365

368

374

375

377

384

386

387

388

389

PAstV 5 HNPDS-01 (OQ781001)

T

G

G

I

I

E

S

N

T

T

S

N

S

E

P

PoAstV-VIRES-JL01 (MK378540)

S

R

G

L

L

H

S

S

S

T

V

S

S

E

T

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

N

R

G

I

L

H

A

S

S

I

T

G

T

E

T

Amino acid sites

390

393

395

400

401

403

405

408

413

416

418

422

432

436

439

PAstV 5 HNPDS-01 (OQ781001)

L

N

L

I

S

P

I

F

V

I

D

A

N

R

T

PoAstV-VIRES-JL01 (MK378540)

Y

H

L

V

P

N

L

L

S

P

A

I

S

N

A

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

S

H

Q

I

P

S

L

L

A

P

A

V

S

S

A

Amino acid sites

441

444

451

—

—

—

—

—

—

—

—

—

—

—

—

PAstV 5 HNPDS-01 (OQ781001)

V

I

D

—

—

—

—

—

—

—

—

—

—

—

—

PoAstV-VIRES-JL01 (MK378540)

A

L

E

—

—

—

—

—

—

—

—

—

—

—

—

PAstV 5-JPN-Ishi-Im1-2 (LC201620)

A

L

D

—

—

—

—

—

—

—

—

—

—

—

—