Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

<div>Schematic diagram of the RT-RAA assays for the detection of BCoV N gene. (a) The flowchart outlines the process for the real-time RT-RAA assay. (b) The RT-RAA-LFD test procedure. The fluorescent probe requires the insertion of an antigen marker, FAM, at the 5′ end, and a C3-spacer block at the 3′ end. An antigen marker, biotin, is also inserted at the 5′ end of the downstream primer. The amplified product is labeled with both FAM and biotin. SSB, single-stranded binding protein; FAM-dT, thymidine nucleotide carrying fluorescein; THF, tetrahydrofuran spacer; BHQ1-dT, thymidine nucleotide carrying black-hole Quencher-1; C3 spacer, polymerase extension blocking group; FAM, antigenic marker carboxyfluorescein.</div>

Transboundary and Emerging Diseases

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Figure 1: Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 

Figure 1 | Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 