Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

<div>Procedure for the RT-RAA assays. (a) Procedure for real-time RT-RAA assay. (b) Procedure for RT-RAA-LFD assay. (c) The leading end of the strip carries a gold nanoparticle, with a FAM antibody on the C-line and a biotin antibody on the T line. C, quality control line; T, test line; Anti-FAM, anti-FAM antigen marker; Anti-Biotin, anti-biotin antigen marker.</div>

Transboundary and Emerging Diseases

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Figure 3: Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 

Figure 3 | Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 