Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

<div>BCoV RT-RAA-LFD sensitivity, specificity, and reproducibility assays. (a) Sensitivity assay. A dilution series ranging from 1.46×107–1.46 × 101 copies/μL was used, with water free of DNAzyme serving as a negative control. RT-RAA-LFD was able to detect as low as 1.46 × 102 copies/μL. (b) Specificity assay. RNA or DNA of BCoV, BVDV, BEV, BPIV3, BEFV, and BRV were used as templates. Water without DNAzyme served as a negative control. Only BCoV showed specific RT-RAA-LFD amplification. (c) Reproducibility assay. Reproducibility experiments were conducted using three standard RNA dilutions: 1.46 × 107, 1.46 × 104, and 1.46 × 103 copies/μL.</div>

Transboundary and Emerging Diseases

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Figure 7

Figure 7: Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 

Figure 7 | Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 