Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

<div>BCoV RT-PCR and RT-qPCR sensitivity assays. (a) RT-PCR sensitivity assay. A dilution series ranging from 1.46 × 107–1.46 × 100 copies/μL was used, and water without DNAzyme served as the negative control. The RT-PCR assay was capable of detecting as low as 1.46 × 104 copies/μL. (b) RT-qPCR sensitivity assay. Dilutions ranged from 1.46 × 106–1.46 × 100 copies/μL, and water without DNAzyme was used as a negative control. The RT-qPCR assay could detect as low as 1.46 × 101 copies/μL.</div>

Transboundary and Emerging Diseases

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Figure 8: Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 

Figure 8 | Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus 