Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

Table 2

Real-time RT-RAA optimal reaction system and RT-RAA-LFD optimal reaction system.

Real-time RT-RAA		RT-RAA-LFD
Reagent	Volume (μL)	Reagent	Volume (μL)
A buffer	25	A buffer	25
ddH2O	15.3	ddH2O	15.9
Upstream primer (10 μM)	2.2	Upstream primer (2 μM)	2.0
Downstream primer (10 μM)	2.2	Downstream primer (2 μM)	2.0
Exo probe (10 μM)	0.8	Nfo probe (2 μM)	0.6
Template	2	Template	2
B buffer	2.5	B buffer	2.5
Total	50	Total	50