Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

Table 3

The results of real-time RT-RAA, RT-RAA-LFD, RT-PCR, and RT-qPCR assays in detecting suspected cases of BCoV infection in clinical samples.

Sample	Total	Real-time RT-RAA		RT-RAA-LFD		RT-PCR		RT-qPCR
		Positive	Negative	Positive	Negative	Positive	Negative	Positive	Negative
Fece	162	17	145	16	146	14	148	17	145
Swab	50	11	39	10	40	9	41	11	39
Tissue	30	6	24	5	25	3	27	6	24
Total	242	34	208	31	211	26	216	34	208