Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus

Table 4

Coincidence rate of real-time RT-RAA, RT-RAA-LFD, RT-PCR, and RT-qPCR methods for BCoV detection.

	RT-PCR			RT-qPCR
	Positive	Negative	Total	Positive	Negative	Total
Real-time RT-RAA	26	8	34 (14.05%)	34	0	34 (14.05%)
Positive	0	208	208	0	208	208
Negative	26 (10.74%)	216	242	34 (14.05%)	208	242
Total			Kappa = 0.848			Kappa = 1
RT-RAA-LFD	26	5	31 (12.81%)	31	0	31 (12.81%)
Positive	0	211	211	3	208	211
Negative	26 (10.74%)	216	242	34 (14.05%)	208	242
Total			Kappa = 0.901			Kappa = 0.947