Effect of PCV-2 Vaccination on Cytokines Gene Expression Profile in Wild Boar Peripheral Blood Mononuclear Cells after Stimulation with Mycobacteria Antigens

Table 2

ELISA or PCR PCV-2 positive animals ∆∆Ct means in the function of cytokine (IL-1β, IL-2, Il-4, IL-6, IL-8, IL-10, IL-12p40, IFN-γ, and TNF-α) or groups of cytokines (proinf, TH1, and TH2), group (NV/V) and type of stimulation (M. bovis, M. avium, and mitogen).

	IL-1β		IL-2		IL-4		IL-6
	NV	V	NV	V	NV	V	NV	V
M. bovis	39.82	2.20	1.62	0.91	1.43	0.99	5.00	0.94
M. avium	2.75	2.76	1.79	0.88	0.91	0.89	1.90	0.98
Mitogen	6.62	0.77	1,319.84	150.11	73.26	21.56	9.95	1.87
	IL-8		IL-10		IL-12p40		IFN-γ
	NV	V	NV	V	NV	V	NV	V
M. bovis	3.79	1.02	4.47	1.66	2.87	0.72	2.12	0.92
M. avium	1.75	1.58	1.51	1.31	17,216.03	2.25	1.35	0.63
Mitogen	3.22	1.34	6.58	1.68	5.98	2.80	17.52	4.92
	TNF-α		PROINF		TH1		TH2
	NV	V	NV	V	NV	V	NV	V
M. bovis	4.91	1.67	49.73	4.81	6.61	2.59	5.55	2.65
M. avium	1.14	1.07	5.80	4.81	17,219.69	3.77	2.26	1.78
Mitogen	3.92	1.99	20.50	4.63	1,343.34	147.31	77.82	23.24

Statistical significance differences between groups are represented with (p-value < 0.05). Bold values (V) have statistical significant differences with NV.