Transboundary and Emerging Diseases / 2024 / Article / Tab 2 / Research Article
Genetics and Pathogenicity of Influenza A (H4N6) Virus Isolated from Wild Birds in Jiangsu Province, China, 2023 Table 2 Molecular markers of H4N6 AIVs from wild birds in the study.
Protein Amino acid position/motif Phenotypic consequences References Isolates in the study PB2 E158K Increased virulence in mice and increased polymerase activity [34 ] —b E627K Increased virulence in mice and increased polymerase activity [34 ] — D701N Increased virulence in mice and increased polymerase activity [35 ] — PB1 D3V Increased virulence in mice and increased polymerase activity [26 ] V D622G Increased virulence in mice and increased polymerase activity [27 ] G PB1-F2 N66S Increased virulence in mice [28 , 29 ] S HA (H3 numbering)a E190G Increased virus binding to human-type receptor [36 ] — G225D Increased virus binding to human-type receptor [37 ] — Q226L Increased virus binding to human-type receptor [18 ] — G228A/S Increased virus binding to human-type receptor [18 ] — T187P + M227L Increased virulence in mice [38 ] — NA (N2 numbering)a Stalk deletion Increased virulence in mice [39 ] — E119A/D/G Reduced susceptibility to zanamivir [40 ] — R292 K Reduced susceptibility to zanamivir [40 ] — M1 N30D Increased virulence in mice [30 ] D I43M Increased virulence in mice [31 ] M T215A Increased virulence in mice [30 ] A NS1 80–84 Deletion Increased virulence in mice [41 ] — P42S Increased virulence in mice [32 ] S I106M Increased virulence in mice [33 ] M
a The mutations/motifs are numbered according to alignments with A/Aichi/2/1968(H3N2). b The “−” indicates the mutation was not detected in the H4N6 viruses.
