Serological Surveillance and Risk Factor Analysis for Parrot Bornavirus in Taiwan

Figure 1

Validation of serological diagnostic measures for this study. (a) One microgram of sample buffer-treated PaBV-4 or PaBV-5 virions was electrophoresed and transferred to a nitrocellulose membrane for the IB assay. After blocking with skim milk, the membrane was incubated with positive or negative parrot serum at 1 : 100 dilution. (b) For the ICC assay, PaBV-4 NTUCL7 strain-infected monolayer QM7 cells were incubated with positive or negative parrot serum to identify PaBV-infected QM7 cells. (c) To verify the consistency of IB and ICC detection results, 40 seropositive and 40 seronegative samples were used to validate the two serological diagnostic measures. (d) The bar chart demonstrates the antibody titer distribution for PaBV-positive serum samples. Each bar indicates the number of positive samples for each titer.

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