Predicting Potential PRRSV-2 Variant Emergence through Phylogenetic Inference

Figure 1

Conceptual framework of data generation for systematic predictive modeling. (a) Temporal distribution of PRRSV-2 L1 ORF5 sequences used in this study. As an example, observation time (t) is shown in July 2011 (vertical arrow) with its corresponding pretree (purple bars) and posttree (purple and gray bars) periods. The pretree and posttree were built for each t set as every six months (red bar) from 2011 to 2020. (b) Example pre- and post-timed phylogenetic trees inferred from sequencing data. Tips in purple show sequences from the pretree that are present in both posttrees. (c) Information computed in an example pretree, including designated variants (colored rectangle frames) and early indicators (red circle shows the ancestral node of the blue variant). (d) Success measures (colored oblong shape) are calculated from variants’ new descendants in the posttree.

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