Molecular Prevalence and Phylogeny of Tick-Borne Viruses in Meat and Dairy Products in the Republic of Korea

Table 1

Selected real-time RT-PCR primer and probe sets for SFTSV, TBEV, and CCHFV detection.

Target	Primers/probes	Oligonucleotide sequence (5ʹ→3ʹ)	Genome position	Amplification conditions	Reference
SFTSV	F1	TGTCAGAGTGGTCCAGGATT	45–64	95°C for 30 min, 95°C for 15 min, repeat 45 cycles of; 94°C for 15 s, and 60°C for 60 s	[24]
	R1	ACCTGTCTCCTTCAGCTTCT	162–181
	P1	FAM-TGGAGTTTGGTGAGCAGCAGC-BHQ	69–86
TBEV	TBE F	TGGAYTTYAGACAGGAAYCAACACA	2,966–2,990	50°C for 30 min, 95°C for 10 min, repeat 40 cycles of; 95°C for 15 s, and 60°C for 45 s	[25]
	TBE R	TCCAGAGACYTYGRTCDGTGTGGA	3,064–3,041
	TBE probe	FAM-CCCATCACTCCWGTGTCAC-BHQ	3,014–2,996
CCHFV	RWC F	CAAGGGGTACCAAGAAAATGAAGAAGGC	1,047–1,074	50°C for 20 min, 95°C for 15 min, repeat 44 cycles of; 95°C for 10 s, 54°C for 20 s, and 72°C for 15 s	[26]
	RWC R	GCCACAGGGATTGTTCCAAAGCAGAC	1,202–1,227
	SE01	FAM-ATCTACATGCACCCTGCTGTGTTGACA-BHQ	1,172–1,198