Molecular Prevalence and Phylogeny of Tick-Borne Viruses in Meat and Dairy Products in the Republic of Korea

Table 2

Detection limit comparisons for all real-time RT-PCR primer–probe sets for SFTSV, TBEV, and CCHFV detection.

SFTSV
Target region	Virus titers (TCID50/reaction)	Average Ct value	Target region	Virus titers (TCID50/reaction)	Average Ct value
YG1 S segment	0.01 log	N/D	HB21 S segment	0.01 log	N/D
	0.10 log	N/D		0.10 log	N/D
	1.00 log	37.01		1.00 log	N/D
	2.00 log	35.14		2.00 log	N/D
	3.00 log	33.76		3.00 log	40.29
	4.00 log	29.11		4.00 log	36.95
	5.00 log	25.18		5.00 log	32.28
YG1 M segment	0.01 log	N/D	HB21 M segment	0.01 log	N/D
	0.10 log	N/D		0.10 log	N/D
	1.00 log	38.11		1.00 log	N/D
	2.00 log	36.54		2.00 log	N/D
	3.00 log	32.7		3.00 log	N/D
	4.00 log	29.62		4.00 log	39.27
	5.00 log	26.29		5.00 log	32.43
YG1 L segment	0.01 log	N/D	HB21 L segment	0.01 log	N/D
	0.10 log	N/D		0.10 log	N/D
	1.00 log	N/D		1.00 log	N/D
	2.00 log	33.49		2.00 log	N/D
	3.00 log	30.87		3.00 log	N/D
	4.00 log	27.62		4.00 log	37.93
	5.00 log	24.27		5.00 log	31.80
TBEV
Serial dilution from original RNA stock	Average Ct value
	Target region
	NS1			NS5
NC	N/D			N/D
−5	N/D			N/D
−4	N/D			N/D
−3	37.39			39.92
−2	33.20			34.30
−1	28.97			29.72
0 (original RNA stock)	25.73			26.29
CCHFV
Genome copies/reaction	Average Ct value
	Target primer–probe sets
	Set A		Set B	Set C
NC	N/D		N/D	N/D
10^1	N/D		N/D	N/D
10^2	N/D		N/D	N/D
10^3	37.82		37.90	N/D
10^4	36.94		34.23	N/D
10^5	33.18		31.18	N/D
10^6	30.18		27.96	N/D
10^7	26.24		24.96	43.80
10^8	23.78		21.02	39.05

TCID₅₀, 50% tissue culture infectious dose; Ct, cycle threshold; NC, negative control; N/D, negative detection.