Revealing the Diversity of the Mycobiome in Different Phases of Ticks: ITS Gene-Based Analysis

Table 3

The procedure of PCR in this study.

Gene	Step	Temperature (°C)	Time	Cycles
16S rDNA	Initial denaturation	95	3 min	1
	Denaturation	95	30 s	35
	Annealing	50	30 s
	Extension	72	1 min
	Final extension	72	10 min	1
12S rDNA	Initial denaturation	95	3 min	1
	Denaturation	95	30 s	35
	Annealing	50	30 s
	Extension	72	1 min
	Final extension	72	10 min	1