Research Article
Rapid, Multispecies Detection of SARS-CoV-2 Antibodies via a Meta-Surface Plasmon Resonance Biosensor
Figure 2
Characterization of the SARS-CoV-2 S-trimer and diagrams of the MetaSPR biosensor assay for S-trimer IgG detection: (a) schematic model of the S-trimer protein; (b) representative elution chromatograph of the recombinant S-trimer protein on size-exclusion chromatography. The figures show reducing SDS–PAGE and western blotting analyses of S-trimer; (c) SEC-HPLC analysis of S-trimer purity; (d) determination of the biological activity (EC50) between S-trimer and human ACE2-HFc receptor by ELISA; (e) determination of the binding affinity between S-trimer and human ACE2-HFc receptor by FortéBio BioLayer interferometry.
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