Genetic Diversity and Biological Characteristics of H3 Avian Influenza Virus Isolated from China in 2021–2022 Showed the Emerging H3N8 Posed a Threat to Human Health

Figure 5

Replication and virulence of H3 viruses in mice. Viral titers in organs of mice after inoculation with 10⁶ EID₅₀ of different viruses. The weight and mortality of the mice were monitored over a 14-day period. Three mice from each group were euthanized at 3 dpi, and viral titers in the organs were determined in eggs. Error bars represent standard deviations. The values labeled with one or two asterisks mean the virus was only detected in the organ of one or two mice, and the dashed lines indicate the lower limits of virus detection.