The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils

Table 1

The killing capacity of the H₂O₂/MPO system. The killing capacity of the H₂O₂/MPO system, both from the RLU and CFU analysis, was calculated by setting the values from the bacterial well as a 0% of killing.

The killing capacity of the H2O2/MPO system	Killing (%)
	0 min		30 min		60 min		90 min
	RLU	CFU	RLU	CFU	RLU	CFU	RLU	CFU
MPO 1 μg/well	0	0	0	1.3	0	0	0	0.6
H2O2 30 μM	29.4	15.8	81.1	77.2	82.9	60.3	46.0	29.3
H2O2 125 μM	29.4	23.1	86.5	84.2	91.4	79.8	78.0	61.4
H2O2 500 μM	69.1	80.6	98.6	98.0	99.5	98.8	99.9	99.6
H2O2 30 μM + MPO 1 μg/well	72.9	79.5	97.6	97.2	99.1	98.0	99.6	98.7
H2O2 125 μM + MPO 1 μg/well	82.4	89.0	100	99.6	100	100	100	100
H2O2 500 μM + MPO 1 μg/well	93.2	96.0	100	100	100	100	100	100