Real-Time G-Protein-Coupled Receptor Imaging to Understand and Quantify Receptor Dynamics
Figure 1
Characterization of the D2LR-YFP fusion protein. HEK-293T cells were transiently transfected with 1 μg of cDNA corresponding to D2LR or D2LR-YFP and used 48 h posttransfection. (a) ERK1/2 phosphorylation levels in cells treated with 100 nM quinpirole for 5 min (% with respect to vehicle treated cells). (b) cAMP levels in cells stimulated with 3 μM forskolin in the absence or in the presence of 100 nM quinpirole (% with respect to forskoline treatment). In (a) and (b), values correspond to the mean ± SEM of three experiments performed in duplicates.
