Real-Time G-Protein-Coupled Receptor Imaging to Understand and Quantify Receptor Dynamics
Figure 2
Measurement of fluorescent intensity in the plasma membrane at a single time point. (a) A stack of images from D2LR-YFP-expressing cells was captured, and one plane was selected for analysis (see Section 2.7.1). (b) One-pixel-width line traced along the plasma membrane over the points with the highest fluorescence intensity. (c) Resulting mean fluorescence intensity in a 5 × 5 pixel area around each of the data points in (b). (d) Linear representation of the measure of the mean fluorescence intensity from the plasma membrane detected contour.
