Real-Time G-Protein-Coupled Receptor Imaging to Understand and Quantify Receptor Dynamics

Figure 5

D_2LR vesicle trafficking. D_2LR-YFP expressing cells were recorded for 6 min. Quinpirole (100 nM) was added 30 s after the beginning of the experiment. A stack comprising the central part of the cells was used for analysis (a and d). Vesicle position was marked on the first frame of the stack to track it over time (b and e). Spatial deconvolution allowed to calculate the distance covered by the vesicles in medium- (c) and quinpirole-treated (f) cells. Analysis of the mean distance covered by all analyzed vesicles (medium 𝑛=23, quinpirole 𝑛=17) indicated similar behavior in untreated and in agonist-treated cells.

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