Real-Time G-Protein-Coupled Receptor Imaging to Understand and Quantify Receptor Dynamics
Figure 6
D2LR-YFP containing vesicle characterization. HEK-293T cells expressing D2LR-YFP (a) or YFP (b) were treated for 6 min with 100 nM quinpirole. In (a) D2LR-YFP was detected by monitoring its own fluorescence (green) and, top to bottom, two representative images from different preparations showing (red) of GM130 (Golgi marker), of transferring receptor (endosomal marker), and of LAMP1 (lysosomal marker) are given. Cell nuclei were stained with Hoescht (blue). In (b) nuclear subcellular distribution of YFP (green) is shown in two representative images from different preparations. Scale bar: 10 μm.
