Real-Time G-Protein-Coupled Receptor Imaging to Understand and Quantify Receptor Dynamics
Figure 8
Effect of agonist treatment on D2LR membrane expression. HEK-293T cells expressing D2LR-YFP were treated for 180 min with 100 nM quinpirole (a) or 50 nM dopamine (b). D2LR-YFP was detected by monitoring its own fluorescence (green). Cell nuclei were stained with Hoescht (blue). For each condition two representative images from different preparations are shown. Scale bar: 10 μm.