Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning

Figure 1

Gadolinium chloride (GdCl₃) administration is associated with suppression of Kupffer cell functioning in rat liver. Kinetics of Kupffer cell inactivation after GdCl₃ treatment (time zero) in livers from euthyroid rats by immunohistochemistry using ED2 antibody (a), rate of colloidal carbon uptake (b), and carbon-induced liver respiratory activity (ΔQO₂) (c) assessed in isolated perfused livers at 72 h after GdCl₃ treatment. ΔQO₂ was calculated by integration of the area under the O₂ consumption curves between 30 and 45 min perfusion (c). Values shown represent means ± SEM for 4 rats per experimental group. ^a𝑃<0.05 versus controls assessed by Student’s t-test for unpaired data.

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