Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
Figure 4
(a) Chromatogram of (±)-catechin hydrate (0.13 mg/L), (−)-epicatechin (0.01 mg/L), (−)-epicatechin gallate (0.36 mg/L), and (−)-catechin gallate (0.04 mg/L) in M9 medium supplemented with 1 mM IPTG and 40 g/L glucose and 3 mM eriodictyol; (b) chromatogram of (±)-catechin hydrate (0.04 mg/L), (−)-epicatechin (0.02 mg/L), (−)-epicatechin gallate (0.04 mg/L) in M9 medium supplemented with 1mM IPTG and 10 g/L glucose and 3 mM eriodictyol; (c) chromatogram of (−)-catechin gallate (0.05 mg/L) and unknown compounds in cultures without IPTG; (d) chromatogram of biosynthesized (−)-epicatechin gallate, (0.08 mg/L) in M9 medium supplemented with 0.5 mM IPTG and 1 mM eriodictyol; (e) chromatogram of (−)-epicatechin gallate (0.34 mg/L) production at 1 mM IPTG and 3 mM eriodictyol in M9 medium.
