The Scientific World Journal

Research Article

Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis

Table 1

Primers used for the study.
Primer NameSequences
F3H_(SacI)-FGCG GAG CTC GAG ATG GCG CCA ACA ACA
F3H_(HindIII)-RGAT AAG CTT ACA CTC TCA AGC AAA AAT CTC
DFR_(SacI)-FGCG GAG CTC ATC ATG AAA GAC TCT GTT
DFR_(HindIII)-RGAT AAG CTT TTA AAC CTT GTT GCC ATT
LCR_(KpnI)-FGTA GGT ACC GCC ATG GCA ATG GCA ATG
LCR_(PstI)-RGCG CTG CAG GTG CCT TCA GTT CTG CAA
F3H_(NdeI)-FGCG CAT ATG GAG ATG GCG CCA ACA ACA
F3H_(EcoRI)-RGAT GAA TTC ACA CTC TCA AGC AAA AAT CTC
DFR_(KpnI)-FGCG CCA TGG ATC ATG AAA GAC TCT GTT
DFR_(SalI)-RGAT GTC GAC TTA AAC CTT GTT GCC ATT
LCR_(NdeI)-FGTA CAT ATG GCC ATG GCA ATG GCA ATG
LCR_(XhoI)-RGCG CTC GAG GTG CCT TCA GTT CTG CAA
S a c I - T 7 - r b s - D FR-FGCG GAG CTC CGA TCC CGC GAA ATT AAT
S a c I - T 7 - r b s - D FR-RGAT GTC GAC TTA AAC CTT GTT GCC ATT
SacI-rbs-DFR-FAAC GAG CTC AAG AAG GAG TAT ACA TAT
SalI-rbs-DFR-RGAT GTC GAC TTA AAC CTT GTT GCC ATT
S a c I - T 7 - r b s - L C R - F TCA GTC CGA TCC CGC GAA ATT AAT
X h o l - T 7 - r b s - L C R - R GCG CTC GAG GTG CCT TCA GTT CTG CAA
SalI-rbs-LCR-FAAC GTC GAC AAG AAG GAG TAT ACA TAT
XhoI-rbs-LCR-RGCG CTC GAG GTG CCT TCA GTT CTG CAA
*Sequences in bold are restriction enzyme sites of degenerate primers.