Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins
Figure 3
Effect of pH and temperature on the CeSP activity. (a) Optimum pH for the protease activity was determined in the H-D-Pro-Phe-Arg-pNA hydrolysis in 50 mM acetate/borate/phosphate buffer in the pH range of 2.0 to 11.5 for 20 min at 37°C. (b) For thermal stability studies, CeSP was kept in Tris buffer pH 7.1 for 30 min at different temperatures (20–60°C). Then, enzyme activity in the H-D-Pro-Phe-Arg-pNA hydrolysis was determined at 37°C in the same buffer.
