Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins

Table 1

Typical steps of the CeSP purification.

Step	Protein^1 (mg)	Total activity^2 (U)	Specific activity (U/mg)	Yield^3 (%)	Purification^4 (fold)
Extraction	1,020	179,712	176	100	1
Fractionation	531	110,400	208	61.4	1.17
Hydrophobic interaction	68.0	20,064	295	11.2	1.67
Ion exchange	6.48	18,360	2,833	10.2	16.1
Gel filtration	1.75	14,742	8,424	8.2	47.8

¹Total protein was estimated by the Bradford method. ²One unit (U) of enzymatic activity was defined as the activity that yields 1 μmol of the product per minute under the conditions as described in Section 2.5.³Yield is given as percentage of isolated active material. ⁴Purification factor is calculated using the specific activity data.