Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins

Table 2

Kinetic parameters for hydrolysis of synthetic peptides by CeSP.

Substrate	𝐾𝑚 (μM)	𝑘cat (s^−1)	𝑘cat/𝐾𝑚 (M^−1 s^−1)
H-D-Pro-Phe-Arg-pNA	55.7±6.7	0.0053±0.0002	95.2±9.5
Bz-Ile-Glu(γ-OR)-Gly-Arg-pNA	297±45	0.0091±0.0006	30.6±3.1
H-D-Val-Leu-Lys-pNA	2,09±189	0.031±0.002	14.8±1.0

Hydrolysis of synthetic peptides (0.020 mM to 1.2 mM) by 50 nM CeSP in 50 mM Tris buffer pH 7.1, for 30 min at 37°C, was followed at 405 nm.