CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation

Table 2

Univariate analysis of factors with impact on the CD4+CD25highCD127low Treg frequencies and counts in 84 patients after allogeneic HSCT.

		CD4+CD25highCD127low	𝑃value	CD4+CD25highCD127low	𝑃 value
		% of lymphocytes		×10^6/l
	𝑛=84
Time of the Treg investigation from HSCT (days)			0.56		0.008
		Median		Median
Patient age:
<45 years	𝑛=36	1.87	0.47	20.95	0.94
>45 years	𝑛=48	2.0		23.1
Donor type:
Sibling	𝑛=38	2.14	0.075	24.0	0.17
Unrelated	𝑛=46	1.8		19.8
Transplant conditioning:
Myeloablative	𝑛=46	1.83	0.24	21.75	0.51
RIC	𝑛=38	2.26		20.84
In vivo T-cell depletion (ATG):
No ATG	𝑛=30	2.14	0.33	25.65	0.03
Use of ATG	𝑛=54	1.81		19.15
HLA matching:
Matched	𝑛=60	1.93	0.63	24.5	0.12
Mismatched	𝑛=24	1.98		17.87
Gender matching:
Match	𝑛=45	1.84	0.63	20.25	0.78
Mismatch	𝑛=39	2.05		22.23
CMV serostatus:
Negative/negative	𝑛=22	2.21	0.041	23.86	0.86
Other	𝑛=62	1.81		20.84
History of acute GVHD:
None	𝑛=51	2.05	0.44	19.13	0.74
Grade I–IV	𝑛=33	1.91		23.5
Immunosuppression at time of study:
No	𝑛=53	1.83	0.46	21.27	0.9
Yes	𝑛=31	2.2		21.05

CD: cluster of differentiation; Treg: regulatory T cells; HSCT: haematopoietic stem cell transplantation; CMV: cytomegalovirus; GVHD: graft-versus-host disease.