Localisation of Abundant and Organ-Specific Genes Expressed in Rosa hybrida Leaves and Flower Buds by Direct In Situ RT-PCR

Figure 2

In situ RT-PCR on Rosa hybrida buds and leaves (a) digoxygenilated sections of flower bud, β-actin, (b) digoxygenilated sections of flower bud, Rh FUL gene, (c) biotinylated sections of flower bud, RhFUL gene, (d)–(g) fluoresceinated sections of flower bud, β-actin, (h) fluoresceinated sections of flower bud, RhFUL gene, (i) digoxygeninylated sections of leaf, β-actin, (j) digoxygeninylated sections, RhFUL gene, (k) biotinylated sections, β-actin, (l) biotinylated sections of leaf, RhFUL gene, (m) fluoresceinated sections of leaf, β-actin, (n) fluoresceinated sections, of leaf, RhFUL gene. Bar = 50 μm.