Zinc Oxide Nanoparticles Catalyzed Condensation Reaction of Isocoumarins and 1,7-Heptadiamine in the Formation of Bis-Isoquinolinones

Krishnakumar, Varadhan; Mohan Kumar, Kesarla; Mandal, Badal Kumar; Khan, Fazlur-Rahman Nawaz

doi:https://doi.org/10.1100/2012/619080

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Volume 2012 | Article ID 619080 | https://doi.org/10.1100/2012/619080

Zinc Oxide Nanoparticles Catalyzed Condensation Reaction of Isocoumarins and 1,7-Heptadiamine in the Formation of Bis-Isoquinolinones

Varadhan Krishnakumar,^1,2Kesarla Mohan Kumar,²Badal Kumar Mandal,²and Fazlur-Rahman Nawaz Khan¹

Academic Editor: N. T. Patil, I. Shibata

Received31 Oct 2011

Accepted30 Nov 2011

Published12 Mar 2012

Abstract

The diversified bis-isoquinolinones were obtained in two steps, utilizing homophthalic acid and various acid chlorides providing 3-substituted isocoumarins in the first step which on further condensation with 1,7-heptadiamine involving C–N bond formation from the lactone in the presence of 10 mol% zinc oxide nanoparticles (ZnO NPs) (<150 nm) afforded the desired bis-isoquinolinones in high yield and purity. The synthesized compounds were then characterized using FTIR, ¹H NMR, ¹³C NMR, and HRMS techniques.

1. Introduction

The isoquinolinones form the core structures of plant alkaloids [1] and biologically important compounds [2–5]. In recent decades, isoquinolinones have been used as a precursor of phenylisoquinoline-iridium complex, a high-efficiency red phosphorescent dopant, in organic light-emitting diodes (OLEDs) [6, 7]. There are many reports for their synthesis including cyclization of 2-chlorobenzonitriles and keto esters [8], condensation of 2-(bromomethyl)benzonitriles [9], 2-methylbenzonitrile and 2-methylbenzamide [10], 2-halobenzaldimines and alkynes [11], denitrogenative addition of benzotriazinones and alkynes [12], double metalation of arylbenzamides [13, 14], N-pyridinylphthalimide and alkynes [15], and the metal-mediated synthesis of isoquinolinones has also been successfully emerged [16–18]. In our group, we have already reported the various 3-substituted isoquinolinones from isocoumarins [19, 20]. Most of the above methods have limitations such as long reaction time, low yield, tedious workup procedure, and use of expensive, toxic reagents or catalysts. Indeed, methods involving C–O bond cleavage with subsequent C–N bond formation are emerging as attractive alternatives for isoquinolinones and that nanosized particles are considered to be attractive as catalysts for their greater reactivity, due to high surface area, recovered easily from the reaction mixture and reutilized for further reaction, therefore the method being more economical [21]. The development of efficient methods for their synthesis is of great importance, and we describe an efficient ZnO NPs mediated reaction for the production of bis-isoquinolinones.

2. Experimental

2.1. Materials and Methods

The chemicals and reagents used were purchased from Sigma Aldrich (India) and were used as purchased. Melting points were measured using open capillary tubes and are corrected with reference to benzoic acid. IR spectra (in KBr pellets) were recorded on Nucon Infrared spectrophotometer (India). ¹H NMR (400 MHz and 500 MHz) and ¹³C NMR (100 MHz and 125 MHz) spectra were recorded on a Bruker 400 MHz and 500 MHz spectrometer in CDCl₃ using TMS as a standard. High-resolution mass spectra (HRMS) were obtained using JEOL GC MATE II HRMS (EI) mass spectrometry. The isocoumarins (III) required for the study were obtained following our earlier report [22] from the homophthalic acid (I) and corresponding acid chlorides (II) (Scheme 1).

2.2. Synthesis of ZnO Nanoparticles

The ZnO NPs required for the present investigation were obtained from hydrolysis of zinc acetate using sodium hydroxide as per Hu et al.’s methodology [23] with minor modification as follows. Zinc acetate dihydrate 2.2 g was dissolved in 300 mL of milli-Q water. NaOH 8 g was dissolved in 50 mL of milli-Q water. Then later solution was added dropwise into zinc acetate solution using a peristaltic pump (Revotek, India) and stirred vigorously using a magnetic stirrer for half an hour. The resulting turbid solution was placed on a temperature-controlled water bath at 90°C for one hour, and thus formed white powder was separated, washed with milli-Q water () and with ethanol (), and then subjected to calcination at 300°C for 4 h.

2.3. Characterization of ZnO Nanoparticles

The ZnO NPs prepared were characterized using powder X-ray diffraction (XRD), transmission electron microscope (TEM), and energy dispersive X-ray spectroscopy (EDX). The XRD was performed using Bruker D8 Advance diffractometer with Cu Kα radiation ( = 1.54 Å). TEM images were taken (Philips TEM, Netherlands) by dropping a drop of sample dispersion (1 mL samples added to 5 mL of propanol and sonicated for few minutes) on a carbon-coated copper grid and dried in vacuum. The instrument was operated with an acceleration voltage of 100 Kv. Simultaneously EDX spectrum was recorded at selected areas on the solid surface to obtain the information on surface atomic distribution. Average particle size and size distribution were determined according to CPS Disc Centrifuge particle size analyzer.

2.4. General Procedure for the Synthesis of Bis-Isoquinolinone Derivatives (Va-e)

2.4.1. Method A

The condensation of 3-substituted isocoumarins (IIIa-e) and 1,7-heptadiamine (IV) was carried out in 100 mL round-bottom flask fitted with Dean-Stark trap for water removal and a reflux condenser. The reaction was performed by refluxing 1 mmol of 3-substituted isocoumarins (III) 0.5 mmol of 1,7-heptadiamine (IV) in the presence of catalytic amount of paratoluene sulphonic acid (pTSA) and 20 mL of dry toluene as a solvent. The progress of the reaction was monitored by thin layer chromatography. After completion of the reaction, the solvent was removed under vacuum, the crude product (V) was dissolved in 20 mL of ethyl acetate and washed with water, then the organic layer was passed through anhydrous sodium sulphate, concentrated the solvent, and then it was purified by silica gel column chromatography using ethyl acetate and petroleum ether (15 : 85) mixture as an eluant.

2.4.2. Method B

A mixture of 3-substituted isocoumarins (IIIa-e) and 1,7-heptadiamine (IV) (1 : 0.5 ratio) in dry toluene in the presence of 10 mol% ZnO NPs was heated under reflux condition. The progress of the reaction was monitored by thin layer chromatography. After completion of the reaction, the catalyst was separated by filtration. The solvent was removed under vacuum, and then the crude product (V) was purified by silica gel column chromatography using ethyl acetate and petroleum ether (15 : 85) mixture as an eluant. The purified products (Va-e) were characterized by FTIR, ¹H NMR, ¹³C NMR, and HRMS techniques. The spectral data of compounds (Va-e) are given in Table 4.

3. Results and Discussion

Presently the ZnO NPs required for the investigation were obtained in high purity and yield from the corresponding zinc acetate solution. The XRD pattern of ZnO NPs suggested discrete diffraction peaks at 31.90, 34.40, 36.37, 47.67, 56.71, 62.98, 66.51, 68.05, and 69.21 which can be assigned to (110), (002), (101), (102), (110), (103), (200), (112), and (120) of hexagonal ZnO NPs (Figure 1) and that the lattice constants were in agreement with the standard card (JCPDS no. 00-0361451). The TEM image of ZnO NPs revealed the average particle size of less than 150 nm (Figure 2) and further supported by particle size analysis (Figure 3). The EDX spectrum of the nanosample also confirmed the presence of Zn and O atoms (Figure 4).

The 3-substituted isocoumarins required for the investigation were obtained following our earlier report [22]. And preliminarily, the bis-isoquinolinones V were obtained from the isocoumarins III (1 mmol) and 1,7-heptadiamine IV (0.5 mmol) in the presence of catalytic amount of pTSA and dry toluene using Dean-stark apparatus (Scheme 2; Table 1). However, the reaction required longer time of 24–32 h for completion and the purity of products was not impressive. The formed products were purified and characterized by various spectral techniques such as FTIR, ¹H NMR, ¹³C NMR, and HRMS techniques.

In the search of reducing the reaction time and to improve the yield and purity of the products, it was evidenced that indium trichloride [24] was an effective catalyst; however, due to their cost and toxicity [25, 26], we envisioned the application of an inexpensive and environmentally benign catalysts. The optimization of the reaction was carried out by varying the catalyst, solvent, reactant substituents.

Initially, the ZnO (bulk) was used as a catalyst for the reaction. In our attempt, in the presence of 5 to 25 mol % ZnO (bulk) catalyst, the reaction continued until 36 h without formation of the desired product, V. Then, we investigated the presently synthesized ZnO NPs catalyst in the presence of dry toluene, and it was noticed that ZnO NPs (10 mol%) mediation improved the reaction yield along with reduced time of 10–12 h (Table 1 entry 2).

The optimization of the catalyst was done using the reaction between 3-(4-fluorophenyl)-1H-isochromen-1-one (IIIc) and 1,7-heptadiamine (IV) in the presence of ZnO NPs to afford the product Vc in high yield (Table 1). Further, it was noticed that the effect of solvent plays a role towards reaction time and that in benzene solvent the yield and purity was increased with reduced reaction time period (Table 2).

The effect of substituents on the reaction was also investigated, and it was observed that the rate of the reaction (Table 2) was increased in the presence of electron donating substituents (entry b) compared to electron withdrawing substituents (entries c, d, and e); however, in the case of nitrosubstitution, there is no progression in the reaction, even after the reaction continued up to 36 hours (entry f).

The reusability of the catalyst was investigated by considering the reaction between IIIc and IV to afford Vc. The catalyst was recovered from the reaction mixture easily by filtration after first run. Then, it was washed with distilled water and ethyl alcohol three times and dried at hot air oven for 1 h. The recovered catalyst was unaltered (Figure 5) and used thrice to obtain the desired product without considerable loss of yield 89, 88, and 87%, respectively. It is noteworthy that the yield of the product in the first, second, and third runs was almost the same as that in the fresh catalyst run (Table 1).

A comparison has been made between the pTSA and ZnO NPs as represented in Table 3. The physical data of bis-isoquinolinones are given in Table 4.

The plausible mechanism involving the role of ZnO NPs in the condensation of isocoumarins and diamine to afford the corresponding isoquinolinones are given as in Figure 6. The mechanism gets initiated through the activation of the carbonyl group by the ZnO NPs with the subsequent nucleophilic attack of the amines to form the corresponding I_A amide derivatives which then cyclized again with the mediation of ZnO NPs affording the corresponding hydroxyl derivative I_H which then facilitates the elimination of water to afford the bis-isoquinolinones V, the catalyst regenerated then activate further isocoumarins and the cycle is continued.

3.1. HRMS, FTIR, and NMR Spectra

The HRMS of bis-isoquinolinones V showed a molecular ion peak M+ in the positive mode. The molecular ion peak of Va was observed at m/z = 537.8527, similarly, that of others (Vb-e) were observed at m/z 565.7487, 574.5888, 606.8743, and 696.3511, respectively.

In FTIR spectrum, the bis-isoquinolinones (Va-e) inferred the carbonyl frequency at around 1646-1647 cm⁻¹. The bis-isoquinolinones V are symmetric with respect to the middle carbon of the heptyl group, and hence there are four magnetically equivalent carbons which appeared in the aliphatic region; the corresponding protons resonated at lower chemical shifts. Similarly, the two CH protons of the isoquinolinone rings appeared as singlet in ¹H NMR and the aromatic ring at its 3-position and the other protons of the isoquinolinone ring appeared at higher chemical shifts. The ¹³C NMR peaks appeared between 162.70–162.85 also indicated the symmetrical carbonyl groups of isoquinolinone rings.

4. Conclusion

In short, a green, inexpensive, and efficient method for the synthesis of bis-isoquinolinone using ZnO NPs as a reusable catalyst was established. This new strategy has the several advantages including mild conditions, high yield, efficient, and environmentally benign approach which overcomes the drawbacks of the earlier condensation methods.

Acknowledgments

The author V. Krishnakumar is grateful to VIT University for their financial assistance as research associateship. The authors also thank VIT University for XRD analysis and providing lab facility. They also acknowledge SAIF, IIT Madras, Chennai for providing NMR and HRMS facility.

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Copyright © 2012 Varadhan Krishnakumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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