TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells
Figure 3
The ability of cytokine-treated MTHCs to process DQ-OVA antigen in vitro: (a) the mean fluorescent intensity of FACS analysis shows that MTHCs treated with different cytokines have the different abilities to process DQ-OVA antigen. Data are expressed as the mean ± SE of three independent experiments. ***. (b) The representative images of confocal microscopy show the process of DQ-OVA by MTHCs treated with IFN-γ + TNF-α: (I) MTHCs cultured with GM-CSF only scarcely show any DQ-OVA fluorescent signal on the cell surface. (II) MTHCs treated with IFN-γ + TNF-α have strong DQ-OVA signals on the cell surfaces. (III) The Z-stack image of cross-scanning of the positive cells confirms that the processed DQ-OVA antigen (fluorescent signals) was localized on the cell membranes.
