Research Article

NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity

Figure 4

(a) Activity of NADP-isocitrate dehydorgenase (ICDH), malic enzymes (ME), glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in Arabidopsis seedlings exposed to 100 mM NaCl. (b) Representative agarose electrophoresis gel of the semiquantitative RT-PCR analysis of the cytosolic (At1g65930), mitochondrial/chloroplastic (At5g14590), and peroxisomal (At1g54340) NADP-ICDH genes in Arabidopsis seedlings exposed to 100 mM NaCl. Gel was visualized by ethidium bromide staining, and actin was used as internal control. (c) NADP-ICDH activity in roots and leaves of Arabidopsis seedlings exposed to 100 mM NaCl. Results are the mean of three different experiments ± SEM. *Differences in relation to control values were significant at 𝑃 < 0 . 0 5 .
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