In Vitro Chondrogenesis Transformation Study of Mouse Dental Pulp Stem Cells

Table 1

Primes and the reaction conditions of RT-PCR.

Name	Sense primer	Antisense primer	Accession No.	Prod. length (bp)	Ann. T (^°C)
CD 146	5^′GGACCTTGAGTTTGAGTGG3^′	5^′CAGTGGTTTGGCTGGAGT3^′	NM_023061	479	60
CD 166	5^′AACATGGCGGCTTCAACG3^′	5^′GACGACACCAGCAACGAG3^′	NM_009655	630	61
CD31	5^′GGTCTT GTCGCAGTATCAG3^′	5^′ATGGCAATTATCCGCTCT3^′	NM_001032378.1	355	58
Gapdh	5^′CAACGGCACAGTCAAGG3^′	5^′AAGGTGGAAGAGTGGGAG3^′	NM_008084	717	62
Coll II	5^′TGGTGGAGCAGCAAGAG3^’	5^′ATGGGTGCGATGTCAAT3^’	NM_001113515	398	53
Coll I	5^′GAGTGCTGTGCTTTCTGC3^′	5^′CTCGGTGTCCCTTCATTC3^′	NM_001113515	532	62.8