Are Ancient Durum Wheats Less Toxic to Celiac Patients? A Study of α-Gliadin from Graziella Ra and Kamut

Figure 1

Total gliadin and α-gliadin determination by indirect ELISA. Total gliadin was assessed by a commercial kit (Immunotech); α-gliadin was evaluated using anti-p31–49 (PN3) and anti-p56–75 (CDC-5) mAbs. Results were read off a semilogarithmic calibration curve, constructed as the dependence of measured absorbance values (vertical axis—linear scale) of corresponding calibrators (standard Sigma—gliadin), range 0–1,000 ng/mL (horizontal axis-logarithmic scale); values are reported as mean ± SD from three independent experiments.