Supercooling Agent Icilin Blocks a Warmth-Sensing Ion Channel TRPV3
Figure 4
Block of TRPV3 in keratinocytes. Representative ratio-fluoremetric recordings of fura-2-loaded human primary keratinocytes are shown. Changes in cytosolic Ca2+ levels upon stimulation are depicted as fluorescence ratio () and displayed as a function of time. Transient stimulation by either 300 μM 2-APB (b) or 6 mM camphor (c) results in a Ca2+-influx. In contrast, stimulation by 10 μM icilin had no effect on the intracellular calcium concentration (a). The physiological state of the cells was controlled by application of 100 μM ATP at the end of the experiment. Upon coapplication of icilin induced Ca2+-signals by 2-APB (b) or camphor. (c) were dramatically reduced, respectively. Bars above denote the application points of the stimuli (black: stimulus; grey: coapplication of icilin).
