PARP Inhibition Attenuates Histopathological Lesion in Ischemia/Reperfusion Renal Mouse Model after Cold Prolonged Ischemia

Figure 2

Morphological kidney injury after ischemia/reperfusion (IR) in different groups and subgroups of C56BL/6 mice. (PAS, original magnification 20x). Note loss of brush border and increased acute tubular necrosis in Parp1^+/+ mouse kidney (IR + 48 h UW subgroup) but only tubular vacuolization in Parp1^+/+ mouse kidney (IR + 48 h UW&DPQ subgroup). Kidney structure was preserved in all subgroups of Parp1^0/0 and in Parp1^+/+ wild-type mice pretreated with ip DPQ. Ultrastructural study confirms higher tubular injury in kidneys immersed for 48 h at 4°C in University of Wisconsin (UW) solution (asterisk). Arrows indicate endothelial cell injury in peritubular capillaries (original magnification ×4600).