Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in Cactus Copiapoa tenuissima Ritt. forma mostruosa

Table 4

Influence of ABA and sucrose contents on indirect somatic embryogenesis of C. tenuissima Ritt. formamonstruosa.

Medium	ABA (μM)	Sucrose (%)	Calli fresh weight increase (g)	Calli with somatic embryo (%)	Number of somatic embryos
					Total	On one cultured callus
MS 0ABA S1^1	0	1	2.12 aB*	75	25	2.3 aB
MS 0ABA S3	0	3	8.94 aA	100	195	17.7 aA
MS 0ABA S5	0	5	10.57 aA	100	202	18.4 aA
MS 0.1ABA S1	0.1	1	1.45 aB	92	49	4.5 aB
MS 0.1ABA S3	0.1	3	10.70 aA	100	173	15.7 aA
MS 0.1ABA S5	0.1	5	10.76 aA	100	250	22.7 aA
MS 1ABA S1	1	1	0.96 aB	100	94	8.5 aB
MS 1ABA S3	1	3	6.48 aA	100	237	21.5 aA
MS 1ABA S5	1	5	10.14 aA	100	258	23.5 aA

 ¹Except ABA and sucrose (S), all media contained constant concentration of the following PGRs: 13.32 μM BA; 16.11 μM NAA; 0.57 μM IAA.
*a: data in columns marked the influence of ABA with the same lowercase letter do not differ significantly at 𝛼=0.05.
A: data in columns marked the influence of sucrose with the same uppercase letter do not differ significantly at 𝛼=0.05.