Research Article

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Figure 3

ANS fluorescence spectra of the acid-denatured GA in the absence (thick curve) and the presence of 2.6 M GLC (dashed curve), 1.3 M TRE (dotted curve), 8 M GLY (dashed with one dot curve), and 8 M EG (dashed with two dots curve). The spectra were recorded at 25°C, using a protein concentration of 0.26 μM and ANS : protein molar ratio as 70 : 1.
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