Chitinase Genes LbCHI31 and LbCHI32 from Limonium bicolor Were Successfully Expressed in Escherichia coli and Exhibit Recombinant Chitinase Activities

Table 1

The primers used in cloning the LbCHI31 and LbCHI32 genes into prokaryotic intracellular and extracellular expression vectors.

Primers names	Primers sequences (5′-3′)	Underlined enzymatic sites
inCHI31-R	ATCGGGTACCAGGAGCAGTGCGGTTCTCAAGCCGGT	KpnI
inCHI31-L	CGATGAGCTCAGCAAAAGGCCTCTGGCTATTGC	SacI
inCHI32-R	ATCGCCCGGGCTGGACCTGACGGACCAGCTCGTT	SmaI
inCHI32-L	CGATGGATCCTGTGACGATGCAGAGCCGGATGGGTT	BamHI
exCHI31-R	ATCGGAAGACTGCATGAAAACGACACTCATCCTAACCG	BbsI
exCHI31-L	CGATGGTACCTCAAGCAAAAGGCCTCTGGCTATTG	KpnI
exCHI32-R	ATCGCC ATG GGGAGGCATTGGAGACTGGTAATC	NcoI
exCHI32-L	CGATGGTACCTCAATTATGACGATGCAGAGCCGGAT	KpnI