The Ethanol Extract of the Inner Bark of Caesalpinia pyramidalis (Tul.) Reduces Urinary Bladder Damage during Cyclophosphamide-Induced Cystitis in Rats
Figure 3
Light microscopy of the bladders of rats with cystitis. Original magnification of 10-fold (Panels (a), (c), (e), (g), and (i)) or 40-(Panels (b), (d), (f), (h), and (j)); hematoxylin and eosin staining. Panels (a) and (b) show control saline + vehicle rats with normal tissue histology. Panels (c) and (d) demonstrate that injection of cyclophosphamide induced mucosal erosion and ulceration, severe edema in the submucosal region (), hemorrhagic foci (red arrows), and leukocyte infiltration (black arrows). Panels (e) and (f) indicate that EECp, at 100 mg/kg, reduced the cyclophosphamide-induced morphological alterations, affecting mainly the leukocyte infiltration and mucosal erosion/ulceration, which was not so clearly observed in the urinary bladders of EECp (400 mg/kg)-pretreated rats (Panels (g) and (h)) that still presented mucosal erosion, ulceration, edema in the submucosal region, and hemorrhagic foci. A slight leukocyte infiltration reduction was observed in these animals ((g) and (h)) when compared with animals pretreated with vehicle and injected with cyclophosphamide ((c) and (d)). Panels (i) and (j) show that mesna pretreatment almost completely reverted the damage induced by cyclophosphamide.
