Research Article

Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract

Figure 2

Electron transmission microscope images taken of S. aureus ATCC-33591 cells not treated; (a)–(c) show distinct OM: outer membrane, PM: plasma membrane, R: ribonucleus, and S: septa of the bacteria. Treated with hydroalcoholic extract of Hymenaea stigonocarpa in half of minimum inhibitory concentration; (d)–(f) show indentation and hole formation appearances (PA), mesossomes (M), thickening of the outer membrane (TO), and septum thickness distribution (ST). Treated with minimum inhibitory concentration; (g)–(k) show condensing of the ribonucleus (CR), detachment of outer membrane (DOM), thickness distribution septa (ST) and aberrant division septa (ADS), ghost cells (GC), mesossomes (ME), and coagulated DNA (CD). Magnification of (a), (b), (c), (e), (j), and (k) is 30000x and Magnification of (d), (f), (g), (h) and (l) are 65000x.
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