Research Article

Overexpression of luxS Cannot Increase Autoinducer-2 Production, Only Affect the Growth and Biofilm Formation in Streptococcus suis

Figure 4

Quantitative determination of biofilm formation of the different strains. All strains were cultured in THB medium supplemented with 1% fibrinogen. Each strain was tested in 8-wells of a 96-well microtiter plate. Time course of biofilm formation of the different strains for 24 h or 48 h. HA9801, ΔluxS, CΔluxS, and luxS+ refer to the WT strain, the luxS mutant strain, the complementation strain, and the overexpression strain. The biofilm formation by the ΔluxS was significantly decreased compared to that of the WT strain ( ). The luxS+ overexpression strain was slightly increased to those of the WT strain and CΔluxS. Negative control (NC) wells contained broth only. The columns represent the means and standard deviations of four or more experiments. The asterisk showed significant difference ( ).
924276.fig.004