Expression Profiling Using a cDNA Array and Immunohistochemistry for the Extracellular Matrix Genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in Colorectal Carcinoma Progression and Dissemination

Table 12

Real-time PCR and immunohistochemistry correlation of differentially expressed extracellular matrix genes in 114 patients with colorectal adenocarcinoma, 𝑃<0.05.

Genes	Classification	RT-PCR	RT-PCR	Parameters analysed	IHC	IHC
		Fold change	𝑃 value		Validation	𝑃 value
MMP-2	ECM proteases	2.17	0.01	Mucinous × tubular	No	0.001
		−1.2	0.04	Peritumoral lymphocyte infiltrate +/−	No	1.000
		−2.11	0.03	Age (>60 yr × <60 yr)	No	0.000
FN-1	Other adhesion molecules/collagens and ECM structural constituents	−3.07	0.02	AGE	No	1.000
ITGB-5	Transmembrane molecules/cell-matrix adhesion	2.11	0.04	Grade cell differentiation: (I, II × III, IV)	No	0.394
		1.33	0.02	TNM (I, II × III, IV)	Yes	0.000
ITGA-3	Transmembrane molecules	2.58	0.01	TNM (I, II × III, IV)	Yes	0.000
MMP-9	ECM proteases	1.13	0.01	Villous × tubular	Yes	0.000

IHC: immunohistochemistry; RT-PCR: reverse transcription polymerase.