Characterisation of Drosophila Ubx^CPTI000601 and hth^CPTI000378 Protein Trap Lines

Figure 3

The patterns of Ubx expression in the T3 haltere and leg discs. (a) The expression patterns of endogenous Ubx in T3 haltere: p = pouch, h = hinge, and n = notum. (b) The expression pattern of Ubx-YFP in haltere and (c) at higher magnification. (d) The expression patterns of the endogenous Ubx in T3 leg disc. (e) The expression pattern of Ubx-YFP in T3 leg disc and (f) at higher magnification. The expression pattern of Ubx-YFP at high magnification shows that the protein trap is nuclear. Double staining of the Ubx-YFP and its endogenous protein using α-GFP and α-Ubx, respectively, in the Ubx ^CPTI000601 haltere ((g)–(g′′)) and T3 leg ((h)–(h′′)) imaginal discs shows that they have very similar temporal and spatial expression patterns.