Exploration on Bioflocculation of Nannochloropsis oculata Using Response Surface Methodology for Biodiesel Production

Table 1

Coded values based on the factor at a time experiment for the 5 variables employed in the study.

Code	Variables	−2	−1	0	+1	+2
𝑋1	Temperature (°C)	20	25	30	35	40
𝑋2	pH	6	7	8	9	10
𝑋3	Flocculation time (hr)	2	4	6	8	10
𝑋4	Bioflocculant size (mL)	0.1	0.2	0.3	0.4	0.5
𝑋5	Cationic inducer concentration (mM)	0.01	0.02	0.03	0.04	0.05