Humanin Rescues Cultured Rat Cortical Neurons from NMDA-Induced Toxicity Not by NMDA Receptor
Figure 5
(a) Effect of humanin on NMDA triggered dynamic changes of cytoplasmic Ca2+ concentration. The fluorescence intensity which is related to cytoplasmic Ca2+ concentration was captured and recorded every 660 ms for 300 successive measurements (for about 198 s) for each subject. 5 to 10 neurons were measured in every group. The curves are formed by the connection of 300 data which stand for the average instantaneous fluorescence intensity of each group. HN only: the addition of 10 μM HN itself in the medium. HN (0.1 μmol/L) and HN (1 μmol/L) groups: HN (0.1 μmol/L) or HN (1 μmol/L) in NMDA treatment groups. (b) Effect of humanin on NMDA triggered dynamic changes of cytoplasmic Ca2+ concentration. Picture 1 to picture 9 stand for the typical successive cellular morphological characteristics of HN (1 μmol/L) on NMDA-triggered increase of cytoplasmic Ca2+ concentration.
