Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability

Table 2

Specific activity and kinetics parameters of wild-type enzyme and mutant enzymes.

	WT	V94W	I100F
Specific activity (U mg^−1)
(mmol)
(μmol min^−1)
(s^−1)
/ (mmol^−1 s^−1)

*The activities were tested in 50 mmol pH 8.0 PBS at 40°C. The values represent the means of three independent experiments (mean ± SD).